Figure 4. To enhance cell adhesion and growth on the membrane, novel coatings are investigated. Here, human derived myofibroblast cells have been cultured to induce extracelluar matrix deposition. The images A-C show that the cells have produced a homogeneous and abundant layer of collagen III (green) and fibronectin (red). Cell nuclei are stained blue by DAPI in the merged picture. To obtain the coating, removal of the tissue-producing cells is required. This process, called decellularization, was performed and the result is showed in images D-F. The layer of proteins was retained on the substrate while the cell component was removed (cell nuclei are not visible anymore in the merged picture). The substrate covered with the layer of extracellular matrix can then be used to enhance endothelial cell adhesion and growth.